Composite
Weak p5

Part:BBa_K2918026:Design

Designed by: TUDelft2019   Group: iGEM19_TUDelft   (2019-09-27)


Weak T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 68
    Illegal BsaI.rc site found at 94


Design Notes

MoClo


Source

Φ29 bacteriophage

References